Restriction Digestion Protocol

Check the compatibility of two restriction enzymes and decide which digestion buffer to use (look at the NEB catalog)

Double digest (digest with 2 enzymes at once)

Vol (uL) Reagent
16 ddH2O
7.5 Digestion Buffer 10X
7.5 BSA 10X
40 DNA
2 Enzyme 1
2 Enzyme 2
75 ul Total  
Incubate at 37C for 2 hours

For single digest: alter recipe, perform digest with one enzyme
Then perform PCR cleanup procedure, elute with 40 uL
Then can digest DNA with second enzyme